HistoTechnology facility

Share on LinkedIn

The QIMR Berghofer and The University of Queensland (UQ) Faculty of Health funded HistoTechnology Facility is a service and research laboratory catering to the needs of scientists and postgraduate students within the QIMR Berghofer, UQ and external institutions.

The unit provides training, technical services and consultation on matters relating to routine paraffin and cryo histology, special stains, immunohistochemistry and antibody optimisation, FISH and CISH labelling, tissue preparation and sectioning for transmission electron microscopy and high-resolution digitisation of histology slides.

The main laboratory is located in the QIMR Berghofer Bancroft Centre, Level I, room 114. A satellite laboratory is on Level 5 of The University of Queensland Centre for Clinical Research (UQCCR) building, Royal Brisbane and Women’s Hospital.

Equipment

The facility has a comprehensive range of equipment for paraffin and frozen sample processing and microtomy, automated slide dewaxing and staining.

Specialised equipment includes:

  • Vision Biosystems BondMax auto immunohistochemistry and in situ slide stainer
  • Leica Polycut CM3500 cryo microtome capable of cutting undecalcified bone or whole mouse or rat sections
  • Leica Ultracut UCT ultramicrotome with cryo attachment
  • Nikon Eclipse E800 colour photomicroscope with fluorescence attachment and
  • Aperio Scanscope XT histology slide scanner capable of scanning slides at up to 100 000 dpi. in conjunction with an extensive array of image analysis software.

The facility can supply a broad selection of secondary detection antibodies for peroxidase and alkaline phosphatase IHC, and immunofluorescence studies.

Usage

The facility accepts work requests from all sources on a pay-for-service basis. Charges are dependent on the origin of the work and broadly fall under a three tier system:

  1. The University of Queensland - Faculty of Health, staff and students
  2. QIMR Berghofer staff and students
  3. External clients - full-cost recovery

(All clients pay for any consumables used to complete the work)

Staff

Laboratory Manager: Clay Winterford

Senior Scientific and Technical Officer: Glynn Rees

Scientific and Technical Officers: Sang-Hee Park, Rita Collins, Doug Harbrow

Scientific Officer: Anthony Chan