Cell sorting

Fluorescence-activated cell sorting is a specialised type of flow cytometry which provides a method for sorting a heterogeneous mixture of biological cells into two or more containers, one cell at a time, based upon the specific light scattering and fluorescent characteristics of each cell. As a scientific instrument, it provides fast, objective and quantitative recording of fluorescent signals from individual cells as well as physical separation of cells of particular interest.

Equipment and costs

Moflo High Speed Cell Sorter – $190 per hour

The sorter was installed at in QIMR in 2005 and is configured with 3 excitation lasers and 9 fluorescent detectors, it was upgraded to a Moflo XDP in June 2012.

FACS ARIA III Cell Sorter – $200 per hour

The is configured with 4 excitation lasers and 18 fluorescent detectors, it was upgraded to a FACS ARIA III in May 2013.

This sorter is housed in a Class 1 type enclosure any biohazard material including all human samples is sorted on this instrument.

Costs for commercial users – please contact Facility Manager Grace Chojnowski. (Please note these prices are subject to change.)

Making a Booking

To request an application form please contact either Grace Chojnowski or Paula Hall.

Offline analysis software available

There are 5 offline computers (Mac and PC) with the following software available:

• Cell Quest Pro
• Modfit cell cycle software (Mac and PC version)
• Flow JO
• Winmdi
• FCS Express
• Gatelogic
• Summit
• BD FACS Diva